Identification of Protein (S) in Ischemia Induced PC12 Cells after Induction with Conditioned Media of IMR-32 and U87mg Cells
نویسندگان
چکیده
Hypoxia to the tissue plays a significant role in ischemia. In spite of the development of various in vitro models of neuronal cultures to study the mechanism of ischemia-induced cell death, not much is known about the role of protein expression in ischemia induced pheochromocytoma (PC12) cells. Present study was done to study the expressions of proteins in hypoxia induced PC12 cells and after induction with conditioned media of human neuroblastoma (IMR32) and human glioblastoma (U87MG). We found down regulation of four protein markers with molecular weight of 97, 75, 40 and 14 kDa in hypoxic PC12 cells. Majority of these proteins reappeared after treatment with the conditioned media of IMR32 and U87MG cell lines. Identified proteins will be helpful to understand the mechanism of hypoxia/ischemia induced cell death and also the neuroprotective role of conditioned medium of IMR32 and U87MG cell at the proteomic level.
منابع مشابه
Secretory factors of human neuroblastoma (IMR-32) and human glioblastoma (U87MG) cell lines induce neurite outgrowths in PC12 cells.
Neurite outgrowth is essential for the communication of the nervous system. The rat Pheochromocytoma (PC12) cells are commonly used in the neuronal cell study. It is well known that exogenous stimuli such as Nerve Growth Factor (NGF) induce neurite outgrowth. In the present study it has been investigated whether or not the conditioned medium from human neuroblastoma cell line (IMR-32) and human...
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